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not more than 20 minutes (If the substrate mixture is not a requirement, the time of incubation should be standardized. It is important to note that the mixture should not be exposed to direct sunlight. Finally, add 1000 µL of stop fluid to every pot and slowly hold the plate to ensure sufficient mixing of the solution. Calculation of the optical mass of every well follows which involves using a microplate equipment which has been standardized. Calculation of Results Determination of the results is the last procedure of ELISA protocol which involves calculation of the average absorbance rate for every category of identical samples, controls, and standards. After the calculation, you should create...

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