Effects Of A Hypertonic Solution Of A Greater Concentration Of Solute In The Elodea Plant

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Effects of a hypertonic solution of a greater concentration of solute in the Elodea plant

Osmosis in living beings is an indispensable process which deals with the movement of water through a semipermeable membrane, from an area of low concentration of solutes to a greater concentration to be able to balance. During the osmosis three types of solutions occur: hypertonic, hypotonic and isotonic. This report will focus more on the hypertonic solution that has a greater concentration of solute with respect to its solvent. Likewise, an experiment will be made with the Elodea plant, which are of aquatic, green, measure between between and three centimeters long and contains plant cells. Osmosis in plant cells acts as follows: the passage of water tends to balance the cell by bowing to the isotonic medium, because of this two phenomena can happen: turgidity or plasoMolysis.

What you want to know with this scientific research is what effects will a hypertonic solution on the Elodea plant have? With this knowledge you can know how the Elodea plant reacts to the hypertonic solution and its application in today’s world;For example, a study on Lake Texcoco is being carried out due to its drought and, consequently, water levels have decreased by the evaporation medium and salt concentrations have increased in water.

Theoretical framework

(Ornamental plants, 2018) mention that the Elodea plant is part of the aquatic environment, are fresh water and develop in rivers and lakes. In addition to this they serve to give oxygen to the fish, they are green and their size ranges between 5 and 30 millimeters in length. Another data that should be noted is that the average size of a plant cell is approximately 100 microns.

(Costas, 2019) As noted above, we will work with the mechanism of osmosis, this process occurs when two solutions with different concentration are separated by a semipermeable membrane and spread the lowest concentration liquid through the membraneuntil they can balance them without the need to contribute energy. In other words, if there were two water and salt solutions separated by a semipermeable membrane, (which only allows the water to pass) the water would move from the solution with less concentration to the one with the highest concentration. There are three types of solutions: 

  • The first is hypotonic, which is when the concentration of the solute is lower with respect to its solvent and consequently enters water to the membrane and inflates making it gain weight;
  • The second is hypertonic, this occurs when the solute has a greater concentration in relation to the environment that is compared, therefore, the water comes out and loses weight;
  • Finally, there is the isotonic solution that occurs when the solute and solvent have the same concentration, as a result they are balanced.

(Allot, A & Mindorff, 2015) mention that osmosis can occur in all cells of living beings, since water molecules, despite being hydrophilic, are small enough to pass through the phospholipid bilay.

(Lafer, 2017) comments that when the operation of osmosis occurs in a plant cell, two phenomena can happen the first is called turgencia, which occurs in a hypotonic medium;The cell absorbs water filling its vacuoles, making it bigger. The other process that can be presented is plasoMolysis. This occurs in a hypertonic medium. It is a process of concentration of the protoplasm of the cell of a plant, due to the loss of water in this. This occurs when the external concentration contains a greater number of dissolved molecules and less water per unit volume compared to cell fluid. Plasamolysis can cause the membrane to take off from the plant wall and cause plasmolysis to be irreversibly.

A similar experiment that was performed was that of osmosis with the help of potatoes, where several samples of potatoes were used and underwent different types of solutions with different salt concentrations, to know how they reacted and what kind were, whether isotonic, hypertonicor hypotonic.

Hypothesis and variables

The elodea plant membrane will decrease its size in a hypertonic solution.

Dependent variable

  • Units: Length of the cell membrane of the Elodea plant in microns
  • Measurement instrument: Vernier App Software Pro .3.10

Independent variable: Elodea plant in isotonic solution and hypertonic solution.

  • Measurement instrument: flask
  • Uncertainty: 0.01 ml

Contrald variables: number of measurements and experimentation time (20 min)

  • Measurement instrument: stopwatch
  • Uncertainty: 1 sec (depends on the stopwatch)

 

Results

ELODEA CELL MEMBRANE LENGTH INTACTED IN ISOTONIC AND HYPERTONIC SOLUTIONS

ELODEA CELL LENGTH IN HYPOTONIC SOLUTION WATER WATER IN UM (MICRAS):

  1. 61.86
  2. 56.51
  3. 85.97
  4. 76.6
  5. 89.42
  6. 103.8
  7. 69.81
  8. 60.62
  9. 75.14
  10. 76.6

 

Elodea cell length in NaCl 1m hypertonic solution in UM (microns):

  1. 25.fifteen
  2. 46.35
  3. 42.67
  4. 3. 4.78
  5. 55.58
  6. 36.76
  7. 36.3
  8. 3. 4
  9. 28
  10. 25.4

 

Analysis

In this table we can see that the largest length of a cell in an isotonic solution is 103.8 microns, unlike the hypertonic solution than the largest size it was 55.58 microns in total. The minimum values of the table were 56.51 Reference to the isotonic solution and the smallest value that the hypertonic solution showed was 25.25 microns.

Average membrane length of elodea cells introduced into isotonic and hypertonic solutions

 

Average length of elodea cells in isotonic solution (key water) (microns): 75.633

Average elodea cell length in NaCl hypertonic solution (microns): 36.499

Analysis

In the previous table you could see the averages obtained from the length of the cell in each solution: isotonic and hypertonic. The average isotonic solution was 75.633 microns and the isotonic solution is 36.499 microns in total.

Conclusions

In conclusion, you can affirm the hypothesis that was formulated at the beginning of the report. Which is that the elodea plant membrane will reduce its size in a hypertonic solution. The membrane in an isotonic solution that was 75 was determined to determine the average.633 µm and in the hypertonic environment it was 36.499 µm (decreased) shown a notable comparison between the two, being one larger than the other and thus ratifying the hypothesis. Some limitations presented by the experiment were the rule when making the scale in Logger Pro. On the other hand, several improvements that can be made is to perform the experiment with more time. Also add more samples and thus have greater measurements in Logger Pro. You could also use another type of software. A program was found with the name scion which can prepare an analysis on any type of cell with greater precision of measures and quality. 

Free Effects Of A Hypertonic Solution Of A Greater Concentration Of Solute In The Elodea Plant Essay Sample

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